FLAER binds very specifically to GPI-anchored proteins on mammalian cells. Paroxysmal nocturnal hemoglobinuria (PNH) cells lack these proteins and therefore donít bind FLAER. The reagent is made by labeling an inactive variant of the channel forming protein aerolysin with Alexa 488, a dye which has similar spectral properties to fluorescein.
Many labs now use FLAER routinely for the diagnosis of PNH by FACS. It is known to be especially useful for detecting very small populations of affected cells.
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